image pro plus best parameters for immunofluorescence

The release of cytokines and chemokines in response to cell or tissue damage has been shown to be involved in regulation of mobilization, trafficking, and homing of stem/progenitor cells, with the potential to facilitate tissue repair. Moreover, the limited cell source, cell leakage, and osteophyte formation represent major obstacles to clinical applications of MSCs for IVD regeneration. In fact, the harsh IVD microenvironment negatively influences the survival and function of the transplanted MSCs, impairing their repair potential. Evidence from several studies has indicated that the regenerative effects of transplanted MSCs depend on the potential for differentiation toward nucleus pulposus (NP)-like cells, and the immunomodulatory and immunosuppressive properties via paracrine secretions. Recently, transplantation of mesenchymal stem cells (MSCs) has emerged as one of the most promising therapeutic strategies for IVD degeneration. This pathogenic process is one of the most common causes of low back pain (LBP), resulting in an economic burden and reducing the quality of life of the patients. Intervertebral disc (IVD) degeneration is characterized by a gradual loss of cellular number and function, and a related breakdown of extracellular matrix.

Image pro plus best parameters for immunofluorescence